FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209097
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAT351.0913936E-11145.136021
GACCGTA351.0913936E-11145.136021
GGCGTAC351.0913936E-11145.136021
CAGGTGT151.2256973E-4145.1361
GTGCAGA253.6929123E-8145.1361
GAGTGCC202.1214873E-6145.1361
AGCCGAC100.0070718154145.1361
GAGTCCA202.1214873E-6145.1361
GGCGTGA100.0070718154145.1361
CAGCAGT151.2256973E-4145.1361
CCGGATC100.0070718154145.1361
GCTGCGA151.2256973E-4145.1361
GTGGCAT100.0070718154145.1361
GCCGCGT253.6929123E-8145.1361
AGAGTGA100.0070718154145.1361
GGGACGT100.0070718154145.1361
GGGACGA151.2256973E-4145.1361
CTGCCGA202.1214873E-6145.1361
CGGGAGT100.0070718154145.1361
GACATGA151.2256973E-4145.1361
Produced by FastQC (version 0.11.5)