FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27204680
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGT26786750.0144.562129
GCCGTAT466050.0144.139626
CCCGTAT776200.0144.11726
CATGTAT557700.0144.02026
GACGTAT340000.0143.907716
CCAGTAT592950.0143.821416
CGTATTG8410550.0143.821098
CACGTAT601950.0143.766716
GTAGTAT338900.0143.733056
CCTGTAT647550.0143.718866
GGTATTG4655650.0143.703198
CTAGTAT519550.0143.669546
CGAGTAT319650.0143.634356
GGCGTAT266700.0143.608836
CGTGTAT406950.0143.60566
AGTATTG6424300.0143.590948
GGTGTAT261100.0143.579096
GCTGTAT386700.0143.43916
CGCGTAT438450.0143.424356
GATGTAT305650.0143.334846
Produced by FastQC (version 0.11.5)