FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16849191
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGTT5530100.0144.54918
AGGGTTT16534400.0144.480449
TAGGGTT4573250.0144.447168
GAGGGTT2459100.0143.956138
CGCAGGG266700.0142.765346
CACAGGG417650.0142.75516
AAGGGTT4092200.0142.730388
ACCAGGG394900.0142.460886
CTCAGGG485500.0142.201956
CCAGGGT1759850.0142.078467
CCTAGGG409600.0141.631426
CCGAGGG246100.0141.606616
CCCAGGG517650.0141.451056
CTAGGGT1396800.0141.087927
GCCAGGG318400.0141.078026
GTCCCAG30500.0140.95354
TCAGGGT1693000.0140.580357
CATAGGG364450.0140.498696
CAAGGGT1350100.0140.485767
CCAAGGG396100.0140.473546
Produced by FastQC (version 0.11.5)