FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11974917
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCG400.0145.12571
CACCGAC11717850.0144.933559
CCACCGA3586750.0144.929538
ACACCGA2832150.0144.927668
TCACCGA3319300.0144.902488
GCCACCG779650.0144.780357
CCCACCG980400.0144.772377
AGCACCG464200.0144.759967
TACACCG835250.0144.759867
GACACCG512750.0144.69737
CTCACCG876000.0144.679727
TGCACCG585200.0144.672127
GTCACCG648950.0144.64797
TTCACCG1024400.0144.647457
ACCACCG803350.0144.642277
AACACCG681700.0144.632637
TCCACCG1033550.0144.622477
GCACCGA2019700.0144.620918
ATCACCG779250.0144.612787
CACACCG811350.0144.574257