FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417060
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT351.0913936E-11145.127011
CTTGTGT351.0913936E-11145.127011
CTAGAGA202.122153E-6145.127011
CTAGACT253.6941856E-8145.127011
AGCGTCA253.6941856E-8145.127011
GGAATCA202.122153E-6145.127011
CGTGGAC351.0913936E-11145.127011
AGGTAGA100.0070731365145.127011
GCGACAT151.2260042E-4145.127011
GAGCTCC253.6941856E-8145.127011
AAGAGAA202.122153E-6145.127011
GGAGACT151.2260042E-4145.127011
TGCCAAC550.0145.127011
CGCGGGA202.122153E-6145.127011
TGAAGCA202.122153E-6145.127011
GAGTCGT151.2260042E-4145.127011
ACGCAGA202.122153E-6145.127011
TCCGAGT253.6941856E-8145.127011
GCGAACC202.122153E-6145.127011
GACACGA450.0145.127011
Produced by FastQC (version 0.11.5)