FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31034084
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA30525150.0144.948859
ATGTTTG7812400.0144.290858
CTGTTTG9732100.0144.272258
GTGTTTG4975200.0144.051998
CCTGTTT3108000.0144.034627
TCTGTTT2696850.0143.840097
GCTGTTT1563850.0143.686047
ACTGTTT2432400.0143.589527
AGTGTTT1202400.0143.534237
CGTGTTT1582500.0143.504447
CATGTTT2527200.0143.487297
GGTGTTT839150.0143.023567
TGTGTTT1399600.0142.838537
TTTGTTT2455000.0142.636837
GCATGTT474500.0142.429986
TCCTGTT881200.0142.339436
CAGGTCA2500.0142.09852
CTCTGTT773800.0142.092396
CCCTGTT915900.0142.091836
TACTGTT708950.0142.072146
Produced by FastQC (version 0.11.5)