FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6627835
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGA151.226208E-4145.121781
AGGAGGA151.226208E-4145.121781
GCTGGGA151.226208E-4145.121781
GTGGACT151.226208E-4145.121781
CGGATGA351.0913936E-11145.121781
CGCGGAC500.0145.121771
CCGGAGA202.1226151E-6145.121771
GAGAGAT100.0070739803145.121771
CAGGGCA450.0145.121771
ACGGAGA100.0070739803145.121771
GCAGGGA202.1226151E-6145.121771
GGCCTGT202.1226151E-6145.121771
GGGGGCA253.695277E-8145.121771
CGGGAGA151.230418E-4144.996962
GAGACGT100.0070922007144.996952
AGCGGAG700.0144.992583
GTAGCAG950.0144.992583
TGCAGGC3300.0144.992584
GCGCGCT6503150.0144.90569
CGACGGC4500.0141.770524
Produced by FastQC (version 0.11.5)