FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8078495
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC151.2262931E-4145.119541
GAGCGCA202.122806E-6145.119541
AGACCGA253.6956408E-8145.119541
GTGCGAT100.007074333145.119541
ACGACGA306.475602E-10145.119541
TGCGTGC151.2262931E-4145.119541
CAGCGCC500.0144.996492
ACGCCGA253.7143764E-8144.996492
ACGATGA100.0070922975144.996492
GGCGATC202.1317865E-6144.996492
AGCTACA253.7143764E-8144.996492
CCAGTGA306.511982E-10144.996492
ACGGTGA151.230444E-4144.996492
GGCGTCC7923200.0144.969999
TGGCGTC2499850.0144.96868
CGGCGTC2481100.0144.921658
CTGGCGT912950.0144.883537
CAGGCGT627800.0144.867667
CCGGCGT1102900.0144.804087
AGGCGTC1752050.0144.713338
Produced by FastQC (version 0.11.5)