FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29528260
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTCA29185600.0144.828149
ACTTCTC6744300.0144.232158
CCTTCTC10300550.0144.181348
TCTTCTC7795250.0144.076078
GCTTCTC4518450.0143.76158
CCCTTCT2931000.0140.421237
CTCTTCT2360000.0140.243017
CACTTCT2303450.0139.729877
TCCTTCT3011550.0139.672067
GCCTTCT2393000.0139.293627
ACCTTCT2315850.0139.16647
GACTTCT1032200.0138.6717
TACTTCT2040050.0137.931057
GTCTTCT1622350.0137.806647
ATCGGAT21277850.0136.89777145
TTCTTCT2226200.0136.883457
CGCTTCT1430400.0136.867747
TGCTTCT1330300.0136.839527
GGCTTCT955400.0136.326587
ATCTTCT1956300.0135.86857
Produced by FastQC (version 0.11.5)