FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546893
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG253.6960046E-8145.117771
CACGAGG500.0145.117771
ATGAGCT306.475602E-10145.117771
GCAGAAT351.0913936E-11145.117771
TGACTGG202.1229662E-6145.117771
ACAGGGG400.0144.999442
TATCAGG600.0144.999422
ACTGAGG306.511982E-10144.999422
AGTGACG151.230355E-4144.999422
CCAATGT3188900.0144.940288
TCAATGT2896200.0144.917478
CAATGTT10336350.0144.904139
GCAATGT1747400.0144.84328
GACAATG435750.0144.826197
GTCAATG562450.0144.799227
ACCAATG733350.0144.794857
AGCAATG400500.0144.793447
CCCAATG917700.0144.787177
GCCAATG627800.0144.78477
GGCAATG343850.0144.739567
Produced by FastQC (version 0.11.5)