FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc02_g124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc02_g124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15381367
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCA2050.0145.12391
ACTACCA1050.0145.123891
GCCTATC1350.0144.999342
GATCTGC15146100.0144.978359
CGATCTG4860800.0144.806788
GCGATCT844550.0144.795797
ACGATCT1174700.0144.777227
CGGATCT791500.0144.718437
GTGATCT730750.0144.636087
ATGATCT1002850.0144.631767
TCGATCT1383200.0144.600137
GGGATCT481700.0144.571817
GGATCTG2629000.0144.521678
TGATCTG4141750.0144.466348
CAGATCT1060300.0144.337
GACGATC214150.0144.318026
GAGATCT590550.0144.207557
GATGATC173200.0144.19986
TGGATCT746950.0144.177937
AGGATCT617350.0144.14777
Produced by FastQC (version 0.11.5)