FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282038653
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT19861550.0142.21019
ACGCATA5326950.0139.278958
TCGCATA5031650.0139.000328
TGCGCAT1302050.0138.141147
CACGCAT1685750.0137.965587
CGCTTGA10078000.0137.930088
TTCGCAT1491500.0136.614157
ATCGCAT1483150.0136.459497
TACGCAT1383500.0136.442867
AACGCAT1624050.0136.167927
CTCGCAT1481400.0136.038387
CCCGCAT1772700.0135.537237
CACGCTT788600.0134.329976
CCGCTTG2746050.0133.415777
CGAGCTT763700.0132.701166
GCTACTC1358600.0132.321848
CCTACTC1609900.0130.529398
CCGGGCT5464950.0130.337899
TCCGGGC1610400.0130.007228
TCGCGCA356900.0129.831656
Produced by FastQC (version 0.11.5)