FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28158353
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCT27744150.0144.781319
GCTACTC5484400.0144.714838
TCTACTC8054450.0144.714728
CCTACTC7731300.0144.701198
ACTACTC6524000.0144.680398
ATCTACT2042750.0142.459787
CTCTACT2226000.0142.394827
CCCTACT2243350.0142.266257
ACCTACT1977400.0142.21847
CACTACT2043550.0142.215977
GGTCTAC369150.0142.18546
TCCTACT2086200.0142.182427
AGCTACT1337950.0142.181537
GTCTACT1689900.0142.175227
TACTACT1837000.0142.168087
TGCTACT1529400.0142.083257
TTCTACT2277950.0142.06587
CGCTACT1596150.0142.0467
GACTACT1108850.0142.038577
GCCTACT1595800.0142.01817