FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209097
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC253.6854544E-8145.184561
GTCGCCA500.0145.184562
CGTGATC253.6854544E-8145.184562
GTCTCAG253.6854544E-8145.184561
GCAGTCA253.6854544E-8145.184562
GCCGGGC253.6854544E-8145.184561
GCTGACA253.6854544E-8145.184562
CTGGTCG253.6854544E-8145.184561
GCCTGCG253.6854544E-8145.184562
GGGGTCC253.6854544E-8145.184562
ACGTGGC253.6854544E-8145.184561
AGCGCAG253.6854544E-8145.184562
CAGGATC253.6854544E-8145.184562
AGCTGTC253.6854544E-8145.184561
GGACGCC253.6854544E-8145.184561
GGTGCAC253.6854544E-8145.184562
GGGCCCG253.6854544E-8145.184561
GATGACC253.6854544E-8145.184561
CTCGAGG253.6854544E-8145.184561
GCTACGC253.6854544E-8145.184561
Produced by FastQC (version 0.11.5)