FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27204680
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGT26911700.0144.974469
TCATAGG7939650.0144.948418
GCATAGG4721350.0144.932348
ACATAGG6520900.0144.929968
CGCATAG1470100.0144.760457
ATCATAG1987050.0144.755087
CTCATAG2319550.0144.723437
TCCATAG2102450.0144.712927
AGCATAG1144500.0144.700857
GTCATAG1486450.0144.69967
CACATAG2113050.0144.669727
GCCATAG1431200.0144.668057
TGCATAG1229400.0144.6627
TACATAG1697000.0144.633387
GGCATAG900550.0144.525317
CCATAGG7803750.0144.376318
CCCATAG2379050.0143.755047
GACATAG1104600.0142.97087
TTCATAG2223400.0142.168557
AACATAG1715300.0139.704967