FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16849191
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAAT16784950.0144.12619
GCAAGAA3038900.0143.09928
CCAAGAA5129600.0143.079778
GCCAAGA958450.0142.996497
TCAAGAA4833200.0142.762918
CCCAAGA1582800.0142.474237
CTCAAGA1464350.0141.59227
TCCAAGA1418600.0141.57967
ACAAGAA3986300.0141.557978
CGCAAGA1004800.0140.49127
CACAAGA1334550.0139.642567
TCCCAAG424800.0139.566366
CCCCAAG500400.0139.545996
ATCAAGA1159450.0139.32227
GTCAAGA928500.0139.293587
TACAAGA1092200.0139.132317
TGCAAGA820650.0139.127587
GGCAAGA588050.0139.08817
CTCCAAG427000.0138.983116
GTCCAAG274650.0138.76396
Produced by FastQC (version 0.11.5)