FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11974917
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACA550.0145.181181
GGAGCAG450.0145.181172
GAGAGTA151.2242353E-4145.181172
GGGAGGA151.2242353E-4145.181171
TGTCTGG351.0913936E-11145.181171
GAGGCGA253.686364E-8145.181172
CGCATAT11835250.0144.95599
ACGCATA2788400.0144.882848
GCGCATA2102100.0144.867898
CCGCATA3595050.0144.850898
CTCGCAT988150.0144.749947
TCGCATA3374800.0144.717098
CGCGCAT645500.0144.688817
GTCGCAT597600.0144.688757
ATCGCAT869600.0144.650247
CCCGCAT1135200.0144.64087
GCCGCAT648900.0144.634547
TCCGCAT933250.0144.61147
TGCGCAT563300.0144.593067
GGCGCAT385850.0144.578697
Produced by FastQC (version 0.11.5)