FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc04_g124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc04_g124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417060
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATG202.1220549E-6145.128392
GAGGATC400.0145.128392
GTGCAGC100.0070729363145.128392
GTGCACG202.1220549E-6145.128391
GAGTGTC151.225958E-4145.128392
TAGAGTA100.0070729363145.128391
TGTCACG351.0913936E-11145.128392
AACCGTG400.0145.128391
CATGGAG100.0070729363145.128392
CAGAAGG100.0070729363145.128392
CGTGGCG100.0070729363145.128391
GGCAGTG351.0913936E-11145.128392
GAGGACA202.1220549E-6145.128391
TAGAGGG151.225958E-4145.128391
CAGCGCG100.0070729363145.128392
AACCGGA151.225958E-4145.128391
GAGTGAC100.0070729363145.128391
GGGTGGC100.0070729363145.128392
TGGAGGG151.225958E-4145.128392
GGCAGAG100.0070729363145.128392
Produced by FastQC (version 0.11.5)