FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31034084
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCT30419200.0144.97529
GCCGGGC5252450.0144.848378
CCCGGGC8928500.0144.832558
CACCGGG2303150.0144.829627
TCCGGGC9030400.0144.829548
ACCGGGC7281000.0144.81318
TGCCGGG1432850.0144.801047
TCCCGGG2616950.0144.79947
AGCCGGG1230300.0144.798867
GACCGGG1239650.0144.788627
CGCCGGG1623500.0144.774497
CTCCGGG2507250.0144.767767
ATCCGGG2310700.0144.767437
GCCCGGG1529550.0144.756327
GTCCGGG1668600.0144.754357
GGCCGGG993350.0144.745187
TTCCGGG2593000.0144.738897
ACCCGGG2161900.0144.731747
CCCCGGG2663600.0144.723857
AACCGGG1805450.0144.70427