FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6627835
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGAC253.6869096E-8145.176072
GATGTGC253.6869096E-8145.176071
GCGACAG500.0145.176071
CGAGTAG500.0145.176071
GCGGTGA253.6869096E-8145.176072
GAGAGGA253.6869096E-8145.176071
TAGCCAG500.0145.176072
GCAGGAG253.6869096E-8145.176071
GTGAGAC253.6869096E-8145.176071
GTGGTAG253.6869096E-8145.176071
CGTCAGG253.6869096E-8145.176072
GAGGGGC253.6869096E-8145.176072
GTGCAGG202.118668E-6145.176061
TGGAGTG100.0070660743145.176062
GAGTGGC202.118668E-6145.176061
TGGAGAG351.0913936E-11145.176062
GGGCTGG100.0070660743145.176062
GGAGAGC100.0070660743145.176062
CACGGAG306.4574124E-10145.176061
GGAGACA151.2243826E-4145.176062
Produced by FastQC (version 0.11.5)