FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8078495
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC151.2246719E-4145.167741
TCAGGCT100.007067315145.167721
ATCGCGC100.007067315145.167721
GCGAGCT100.007067315145.167722
AGCTTGA5395200.0144.912122
AAGCTTG5373300.0144.884061
GAATTCG5557250.0144.75517
TGAATTC5545700.0144.71676
TCATGCG203800.0144.707957
TTGAATT5517400.0144.616845
AATTCGA5585700.0144.599278
ATTCGAA5596450.0144.567649
CTTGAAT5508250.0144.552984
ATGCGAT2401650.0144.4199
GCATGCG129750.0144.377917
CATGCGA772350.0143.847388
ACATGCG189100.0143.84247
ATATGCG162200.0143.785747
GACATGC31750.0143.622516
TATGCGA681700.0143.524958
Produced by FastQC (version 0.11.5)