FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29528260
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAT23603550.0144.559849
CGCTTGA7367300.0144.516638
AGCTTGA11426600.0143.812188
TCGCTTG2137050.0143.337437
CTAAGCT4078400.0143.21775
ACGCTTG1884000.0143.18057
TCTAGCT243100.0143.020285
CCGCTTG2233150.0142.730187
TAGCTTG2005600.0142.668667
TCTAAGC2752500.0142.410724
CTCGCTT623000.0142.153816
CTAGCTT626100.0142.109956
AAGCTTG6598750.0142.09037
GCGCTTG1214200.0142.025477
CTAGGCT105900.0142.006035
TTGCTTG1937450.0141.875957
GAGCTTG977200.0141.839927
CTACGCT177150.0141.826075
CGCGCTT382950.0141.79386
TAAGCTT4951750.0141.734156
Produced by FastQC (version 0.11.5)