FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10546893
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTGT351.0913936E-11145.178512
GAGTCGC151.2243206E-4145.178512
GCGCGGC306.4574124E-10145.178512
AGAAGAC550.0145.178511
GCGCCGT351.0913936E-11145.178511
GCCTGGT351.0913936E-11145.178512
TAGGAGC202.1185479E-6145.178512
GGAGGTC202.1185479E-6145.178511
GCAGGGT500.0145.17851
AGGGGTA500.0145.17852
GAATCGT10379850.0144.977339
AGAATCG2440300.0144.825628
AAGAATC625100.0144.818027
TGAATCG2970400.0144.799188
CGAATCG3165850.0144.781338
TCGAATC868650.0144.7757
GAGAATC393500.0144.77097
ATGAATC746900.0144.759037
CCGAATC977100.0144.739737
GGAATCG1853550.0144.733868
Produced by FastQC (version 0.11.5)