FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n01_bc02_g124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n01_bc02_g124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15381367
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGG351.0913936E-11145.15661
GGCCGGG306.4574124E-10145.15661
ATTGCTG550.0145.15661
GGCCTGG253.6901838E-8145.15662
ATCGAGG500.0145.15661
AGGGCGG100.007068998145.156591
AGTGATT15225100.0144.979059
TAGTGAT4166200.0144.941138
GAGTGAT2292100.0144.91118
AAGTGAT3758050.0144.894968
CAGTGAT5035000.0144.860878
GCCAGTG273550.0144.75486
GTTAGTG215900.0144.72476
CGCAGTG260700.0144.715266
GACAGTG194100.0144.694536
CAAGTGA1200250.0144.685297
GCAGTGA819850.0144.621937
CCAAGTG370800.0144.621866
TGCAGTG208400.0144.610676
CTAGTGA1238500.0144.595297
Produced by FastQC (version 0.11.5)