FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31879076
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1968120.6173704658190219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG134150.017.3692721
TAGGTAG76650.017.112425
TATACAG198450.016.286545
GTATAGA160750.015.8046291
GTATATA344850.014.2082581
ACTATGC165400.013.8430728
TATACGG93100.013.5439265
TCTATAC159150.013.3422333
TAGACCG94750.012.8491715
TACTATG166500.012.7086677
TATATAC374950.012.3121423
TATTAGG145150.012.2327122
ATACAGG136900.012.2279476
GTATAGG127100.012.2218081
CTATGCA129050.012.076519
TATACTG198700.012.0354165
TAATACT307750.011.962814
GTACTAG93500.011.9557371
TACACCG60500.011.8583665
CTATACT163250.011.8085354
Produced by FastQC (version 0.11.5)