FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646986
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG2755.1631196E-621.1157471
CCGCGCG3208.482657E-720.390349
GGGCCGC4303.7533027E-818.5441516
TCCTAGG4003.5724042E-718.1233772
ACTATGC13950.017.6680018
CCGGGCG2550.001592078217.0585869
GACCGCG3453.686457E-516.8094547
CTACACT10850.016.7032594
TATATAC23000.016.3895283
CTAATAC15050.016.3769633
TAATACT23550.016.3145794
CACCCCG4901.6676313E-716.273447
TTAGGGT8500.016.2041264
GTCCTAG4951.8442188E-716.1300851
ACTACAC10800.016.1093653
ATCCTAG6801.1459633E-1016.0114821
GTACCGC6006.146365E-915.7063356
CTATGCA11150.015.6051639
GGTTAGA11650.015.57626151
TATGCAG13500.015.5720925
Produced by FastQC (version 0.11.5)