FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3373896
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG15400.021.1842175
GTATTAG29750.018.7803751
TATACAG37900.017.7895245
GTATAGG21100.016.8505521
CTAATAC30200.016.8039633
CTAGGAC9500.016.7888013
GGTAGTC18750.016.239357
TATACTG40450.016.130385
GTGTAAG18000.016.1245651
TAGTCAG18900.016.1109459
TATTAGG32050.016.0604382
TAGGACA17000.015.7787814
GTCCTAG9800.015.5486881
TATACGG20600.015.4848175
GTATAGA32150.015.3471741
TCTATAC29200.015.1449413
CCTAATA32100.014.9061652
CTTACAC15850.014.6366373
ACTATGC27350.014.578948
CTATGCA25000.014.4998499
Produced by FastQC (version 0.11.5)