FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750958
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1002.7338065E-643.498668
AGTCCGG1402.7463497E-531.0704735
CCGCGCA1902.0759762E-730.525389
CCGGCCC1100.0096715326.3628258
AGGACCG1958.752788E-626.024845
GACCGCG1409.994061E-425.892067
TAGGTAG1902.1929185E-422.8940355
CTACACA2903.2847674E-722.499314
AGACCGC1700.00309263721.3228746
TATCCTA3457.500603E-821.0138455
GTATATA6300.020.7343561
ATACAGC4251.4679244E-920.469966
ACCGTAC2505.9733757E-520.2993768
TAGTCAG2505.9733757E-520.2993769
TACAGCG2155.0555717E-420.2319367
CTATACA3951.4109901E-820.1892534
TAAGACA3951.4109901E-820.1892534
AGCTCGA1800.004306856520.138276
CCGCCCG1800.004306856520.138279
GTAAGGG2908.247187E-619.9993863
Produced by FastQC (version 0.11.5)