FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257757
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10950.035.7528275
GTATTAG19250.030.9182071
TAGTCAG11200.029.1289799
GGTAGTC13100.027.1178687
TATTAGG20350.026.0075132
TTAGGTA17000.024.734884
GTATAGA21150.024.3657151
ATTAGGT19800.024.1662653
CTAATAC21750.021.6663073
TATACAG23000.020.8040035
TCTATAC22900.020.2616733
TACAGCG8950.020.251067
CTTACAC10400.020.216013
GTAGTCA17600.020.1843228
CTATGCA17100.019.9265699
CTGTGCG5153.8198777E-1119.708419
GTATAAG19050.019.4315031
GACTAGG7100.019.4010858
TAATACT32000.019.0309334
TATACTG27350.018.5554495
Produced by FastQC (version 0.11.5)