FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177921
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12850.023.6959785
GTATTAG23750.022.308291
GTATAGA28000.019.4406281
CCTACAC10200.019.1907543
CTAATAC29450.018.7092673
TCTATAC27950.018.6757983
ACTATGC27850.018.2222238
ACTGTGC16100.018.012038
TACACCG12600.017.836915
TATACAG34700.017.7589785
TATACTG38150.017.4832345
TACACTG20950.017.3027235
CTATGCA22050.016.7686049
CTATACA28450.016.5637864
TATACGG17700.016.3838215
GTGTAAG17750.016.3556821
GTATAGG21900.016.238991
TAATACT48050.016.1442874
TATAGTG27900.016.1107585
GTATATA67300.016.068611
Produced by FastQC (version 0.11.5)