FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842410
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11250.028.35425
GTATAAG21600.023.519071
TATACCG12200.023.1751215
GTATTAG21200.022.5935211
CTAATAC27850.021.0851673
TATACAG26050.020.037435
CTATGCA16100.019.363119
TTAGGTA18800.018.8953744
TCTATAC21550.018.8390063
TAGACCG16200.018.7953995
TAATACT37600.018.7025644
ATACCGT19850.018.6263146
TATAAGA29700.018.5516262
TAGTCAG13300.018.5336029
GTATAGG17350.018.4047131
GTCCTAA11850.018.3729441
TAGGACA13100.018.2624864
TATACTG31150.018.1532255
CTAGGAC8000.018.1241323
GTGTTAG17850.017.8891751
Produced by FastQC (version 0.11.5)