FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946683
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13850.025.681671
TAGGTAG8000.022.655255
TAGACCG8750.019.8848385
GTACTAG9200.019.725551
TATTAGG15500.019.6442952
TACACCG5759.094947E-1218.9122095
AGTACCG10750.018.8828875
TAATACT26650.018.4982454
CTAATAC16150.018.404763
GTCCCGG2801.4134607E-418.1475071
CCTATAC11750.017.8928283
GTATAGA14200.017.8919071
TATACAG16650.017.8520665
GTATTAT24350.017.5885491
ACAGTGC8700.017.4992288
TGTAGAC14300.017.2370013
TATACTG20050.016.9942635
GTCCTAG5151.5697879E-816.914181
CTATACT15050.016.8597224
CTAGGGT3453.683022E-516.8108524
Produced by FastQC (version 0.11.5)