FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644739
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC3200.031.7169193
CTTAGGG1402.7462582E-531.0696333
TAGGTCG1453.489868E-529.9982645
CTAATAC6000.027.790063
CTTAAGG2405.268521E-827.1859283
ATACCGT4800.027.1859286
ACCGTTC2954.820322E-1027.0323358
TATACCG3408.913048E-1125.5867565
TACACAG3408.913048E-1125.5867565
CTAGGTC1801.5200635E-424.1652724
GTATAGA5450.023.9714581
GTATTAG4850.023.9439981
TCTATAC5650.023.0960123
CCTATCC1902.1928527E-422.8934153
CCTAATA6550.022.1378422
GTCTTAG2303.1130847E-522.089641
TACAGCG2303.1411902E-522.0639447
ACTATGC6300.021.8638178
ACTAGGC2353.705982E-521.5961729
CTATGCA4751.2732926E-1121.3688439
Produced by FastQC (version 0.11.5)