FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367971
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79030.10726155138232765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC20550.020.4614033
CTATGCA42350.018.6598039
CTAATAC55150.018.5350113
TAGGTAG24400.017.529985
TAGACCG39150.017.4066565
GTATTAG48000.016.9334031
CCTAATA53900.016.8128172
GTATAGG44200.016.7473221
TAATACT84300.016.5977424
GTATAGA52050.015.8946711
TATACAG52400.015.4955155
CTATACT46250.015.2047494
ACTATGC57300.015.0560698
TACACCG21700.015.0339135
TATACGG30050.014.9577565
GTATATA80700.014.74815751
TATACTG70450.014.4067595
AGACCGT46900.014.3757116
GTATATG55150.014.3432791
TCCTAGG13150.014.33398152
Produced by FastQC (version 0.11.5)