FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537094
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6540.12176639470930602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAC1307.2777766E-944.6576841
CTCCGTG657.33632E-444.611957
GATCCGC1105.262533E-639.5424086
GGACTAG800.002026328836.2843671
ATACTAT4000.036.2472086
CAGGACG800.002036547336.2472044
AGGACGG900.003628115432.219745
TCCCTCG950.004727148530.5239647
CCTCTTA1702.9861567E-629.8812431
TCCCTGC1000.006074273528.9977662
ACCCTTG1000.006074273528.9977667
GACGCGT1000.006074273528.9977666
TAATACT5050.028.7106574
TATACCG1804.6974583E-628.1922745
CCTAATA3351.8189894E-1228.1321622
CCCTTAG1306.443204E-427.9110531
CTAATAC3403.6379788E-1227.718453
TACTATG3005.857146E-1026.5812857
GCCCCCT1100.0096231226.388631
CCCTGAG1100.00967130826.3616052
Produced by FastQC (version 0.11.5)