FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4006801
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG31900.017.2914311
TAGGTAG18700.015.8946675
TACACCG15800.015.6002285
TATACAG46950.014.8232975
CTAATAC33150.014.6521193
CCTAGAC15500.014.4990383
GTATTAT60600.014.2522141
GTCCTAA17850.014.2310791
TAGACAG25300.014.0405695
TATACTG49800.013.9749765
CTATACA36050.013.8756384
GTATATG46700.013.8318781
TCTAGAC19050.013.6998783
GTATAGA34100.013.6217731
TATTAGG39300.013.4661942
CTAGACA19500.013.3837264
TAGACCG19550.013.3494975
TTATACT51200.013.3096624
GTATAGG27400.013.2442531
CCTTATA31050.013.0749782
Produced by FastQC (version 0.11.5)