FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037344
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG14350.027.7864955
GTATTAG24650.022.3779541
GTATAGA26850.021.8959831
CAGCGTG12000.021.7496059
GGTAGTC15450.021.5850127
CTAATAC27650.021.2376123
GTCCTAA13800.021.038011
TAGTCAG14500.020.9996179
TCTATAC29050.020.9627863
CCTAATA27000.020.6752092
ACTATGC24950.020.6305488
ACCGTGC7050.020.5666628
TATACAG30800.020.0074235
TAATACT43350.019.5664754
CTATGCA20750.019.565919
GACTAGG10850.019.377218
TACTATG28450.019.366647
TATACCG13700.017.9920775
GTCCAGA19950.017.8269441
ACAGCGT16700.017.7987848
Produced by FastQC (version 0.11.5)