FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470798
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC42100.021.8699763
GTATTAG33950.020.9469721
TAGGTAG19450.020.5005235
CTATGCA32750.019.7022199
ACTATGC38000.017.9333428
TAATACT66500.017.7702144
TCTATAC36450.017.7018723
CCTAATA41550.017.6238732
TATACTG55900.017.3786015
TATTAGG39550.016.6819152
GTATAGA40250.016.0457131
TACTATG47300.015.7869397
TATACGG27100.015.7835465
GTCCTAA20550.015.5373081
ATACTAT64800.015.4392416
TATACAG46700.015.3688095
GTGTAAG22750.015.3106891
ACAGTGC23050.014.7823648
TACACCG14750.014.7452155
TAGTCAG20700.014.7100919
Produced by FastQC (version 0.11.5)