FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5170917
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG23350.025.1488135
GTATTAG43800.022.5324551
TATACAG58000.019.7491535
CTAATAC44850.018.9122333
TAGTCAG24800.018.7094179
GTATAGA46350.018.6312071
TCTATAC45700.016.8154723
GGTAGTC29750.016.5707197
TATACCG28150.016.4824185
TTAGGTA43050.015.8297514
TACAGCG22050.015.4528527
CTTAGAC28300.015.3703663
GTATATA108900.015.3265241
TATACGG29850.015.300855
GTATAGG33200.015.300431
TATACTG64400.014.9721845
ACCGCGC8309.094947E-1214.8486178
CCTAATA50500.014.7864932
GTATATG63800.014.4453081
CTATGCA37700.014.4228779
Produced by FastQC (version 0.11.5)