FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577434
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15790.10009927515192395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13000.019.5338231
TCTATAC11700.019.2085293
TATAAGA17600.018.9480042
AGGGCCG1950.006844063318.5888985
CTAATAC14450.018.563173
CCTAATA12900.018.545672
GTATTAG11750.018.5244771
ACTATGC12250.018.3461048
GTATAGA13500.018.272911
TAGGTAG6900.017.8615075
TAATACT20400.017.4134244
TATACGG8450.017.1589835
AGACCGC4751.16840965E-716.788716
ATACAGT14700.016.7679446
ACCCTGC5652.764864E-916.6806568
TATACTG20450.016.6618355
GTATTAA15700.016.1745011
CCTAGAC6306.7484507E-1016.1103783
GCAGGGC2700.002332897616.1103788
CTAACAC11350.015.9684363
Produced by FastQC (version 0.11.5)