FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128473
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21370.10040061584055798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8800.024.7152775
GTATTAG14650.024.2764681
TATACAG18700.022.0983645
TATACCG8900.021.9938185
ACTAGAC8800.020.5960643
ACCGTGC4601.7826096E-1020.4886058
ATACCGT15900.019.1504546
TATTAGG16700.019.1017552
GACTAGG6101.8189894E-1219.0159078
CTAATAC16850.018.9312653
TAGTCAG8050.018.912569
CTATGCA13600.018.657619
CTAGACA11400.018.442514
GGTAGTC10350.018.2120957
ACTATGC16850.018.0707538
GTATAGA17300.018.0405351
TAGACAG12200.017.2331665
GTAGGAC5051.2518285E-817.2272833
CCTAGGC3002.4185215E-416.9166322
TATACGG11950.016.3803335
Produced by FastQC (version 0.11.5)