FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952782
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41350.43399224586526614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG3902.0008883E-1124.16635
TATACAG6050.023.9665785
CGCACCG2303.1421892E-522.0648829
GTACTGG5600.022.0398241
CGTGCAC1650.002600999821.9693623
ACTGCGT2003.1032076E-421.7496688
ACCTGTG2704.445081E-621.4811557
ACCTCGC1700.003092961221.3232047
TAATACT9600.021.1455124
GCGCACC2104.3143687E-420.7139728
TGTACCG2455.1142786E-520.713975
CATATAC6350.020.5508673
CCTCGCG1800.00430730420.1385828
GTACCGT3259.830146E-720.0766186
CTAAGGT2908.249903E-619.9996954
ACTAGCG2608.077745E-519.5189323
ACAGTGC3001.1055783E-519.333048
ACTGCAC2256.868056E-419.3330383
ACACAGT4152.4900146E-819.2165766
AACCTCG3051.27500825E-519.0161046
Produced by FastQC (version 0.11.5)