FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480253
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18070.12207372658592823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC3457.514063E-821.0135428
CAGCCCG1900.00588958219.0787329
TAACCGG3902.7350143E-718.5889035
AAGACCG7050.018.5098025
CCCTAAG3252.1883912E-517.8610341
GACCGCG3252.2049888E-517.8453487
GTACTAA6102.1827873E-1117.8427331
GTACTAG5952.9467628E-1017.0730481
TAGAAGT10250.016.9748424
GCCAGAC4307.6124707E-716.8597013
CTAATAC9050.016.8224453
AGACCGC4751.1682641E-716.7887156
GCTATAC4358.590541E-716.6659153
GTATAGA7950.016.428781
TATAGTC7151.4551915E-1116.2230435
CTATAGT5402.8592694E-816.1103844
CTAAGCA6306.7484507E-1016.1103844
CTATGCA6851.2914825E-1015.8757319
GGATAGG5052.3219945E-715.8052461
GTATAAT10250.015.573951
Produced by FastQC (version 0.11.5)