FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871827
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7250.026.0330051
CTGATCG2151.8661232E-523.6034379
GTGAGCG1851.8310426E-423.5123049
GTCTAGG3102.3846951E-823.4167841
CAGTCGC1700.003093402621.3224339
TAGGTAG3809.044015E-920.9857625
ACAGTGC3508.7376975E-820.713228
TACACCG3157.283161E-720.713225
GTATAGG5503.6379788E-1219.7978251
TAATACT12650.019.4851654
TATACTG10850.019.3768845
CTGGTCC3401.515109E-619.190194
TATACAG8350.019.1008155
CTAATAC6600.018.6732833
CGGAGCG1950.006837370318.59092145
ATCGTGC2359.201962E-418.5096878
ACTATGC7500.018.365728
TATACCG3953.1232048E-718.3534875
CTCTAGA5157.894414E-1018.3242021
CTAGACA4908.60382E-917.7541894
Produced by FastQC (version 0.11.5)