FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73650.23793539916611156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA28050.019.38432
CTAATAC27850.019.2631913
CTATGCA20900.018.731969
TAGGTAG15650.018.5296555
TATACAG32450.017.2027455
ACTATGC28000.017.0886448
GTATAGA26850.017.0237351
GTATTAG26050.016.9895021
TATTAGG27700.014.6564892
CCTAGGC4051.4482718E-414.320453
TATATAC56150.014.0733813
ACCTAAT33250.013.9651911
TACAGCG12550.013.8640217
TAATACT44650.013.8012734
CTATACT24050.013.564984
TAGTCAG14500.013.4999299
TACTATG31400.013.3912187
ATACTAT45100.013.3420716
TCCTAGG8201.9390427E-913.2616982
CCCTATA14950.013.0931542
Produced by FastQC (version 0.11.5)