FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612840
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG2451.9736035E-929.5915459
CTATGCA7850.027.7067389
ACTATGC9700.024.6639428
GTATTAG8700.024.1934321
TCCCGCG2502.2814693E-623.1990537
CCGCGCC1600.00217584922.6560279
TAGGACA5450.022.6137564
ACACCGA4607.2759576E-1222.0643166
TAATACT16400.021.66074
ATACTAT13850.021.4612186
TACTATG10500.021.4038877
AATACTA14700.021.2066175
CCTAATA9400.020.824262
CTAATAC10650.020.42173
GTATAGA8450.019.7555831
CTATTAG10500.019.3547461
ACCTAAT10900.018.644481
TTAGGCA5107.075869E-1018.4796374
CTAGAGT5601.2551027E-1018.124264
CCCGCGC3602.619725E-618.124268
Produced by FastQC (version 0.11.5)