FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4897218
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG22500.021.2654385
GTATTAG39750.020.9963551
CTAATAC45200.018.605123
ACTATGC39950.018.3281028
TATACAG54250.018.1740655
CTATGCA35250.017.4821539
ACCGCGC7150.017.2367678
TAGACCG23700.017.1298125
CCTAATA45950.016.8816782
GTATAGA42100.016.7214071
TCTATAC43450.016.3511853
TACTATG48900.016.3078527
TATACTG58800.016.274575
GTCCTAG13800.015.7770341
CTATACA43450.015.6837894
TATACGG31450.015.674775
TAGTCAG23850.015.1990599
GTATATA101500.014.9438831
CTTACAC25000.014.7891463
GGTAGTC26650.014.6895847
Produced by FastQC (version 0.11.5)