FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497356
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6860.13792937051126355No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC850.00274088134.1171048
CTAGTCC850.00274088134.1171043
TAGTCAG1504.394794E-528.9995389
GCGTGCA1000.00607203428.9995379
CTAGGCC1000.00607203428.9995373
GGTGCGG1555.495543E-528.0640669
CCTAATA2405.261245E-827.1870672
AATGCCG1358.077724E-426.8514235
ATAGTAC2207.4743184E-726.3632163
CCTATGA1450.001225690124.9996012
CATGCAC1751.254621E-424.8567473
TGTACGA1751.254621E-424.8567472
TATACGG1801.5189512E-424.1662835
ACTCCGC1500.001493357824.166288
TCTATAC3301.7680577E-924.166283
ATCCAGA3652.1645974E-1023.8616261
GTCCTAA2151.847631E-523.6304071
AGGGATG1550.00180738523.3867235
TATAGAA4700.023.137932
TCCAGAG3203.3774995E-822.6558882
Produced by FastQC (version 0.11.5)