FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4418361
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG20300.019.2848115
GTATTAG35250.017.7039451
GGTAGTC20950.016.61027
TATACAG49500.016.549735
TATTAGG36800.015.7606692
TAATACT68350.015.6976984
TATACGG27800.015.646755
TAGTCAG21550.015.4750899
GTATAGA38650.015.0200451
GTATATA89200.014.8059841
TATACCG23550.014.776385
CATATAC55750.014.6942133
CTAATAC39150.014.2584313
TAGACCG20850.014.2559285
ACTATGC35400.014.1305358
TATATAC98850.014.0811243
CTATGCA29100.013.9513959
AGGACCG11600.013.7493575
ATACAGG31300.013.6654956
TAGACAG24950.013.6566765
Produced by FastQC (version 0.11.5)