FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n02_Fluc_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n02_Fluc_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258269
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA35700.020.329931
TAGGTAG18650.020.2140855
GTATTAG34850.019.3679771
CTAATAC38700.018.3588163
TATACGG24900.017.469545
TACACCG16250.017.3996625
GTATAAG36200.017.242251
CCTAATA39700.016.8008792
CTATGCA29600.016.4101549
TAATACT61950.016.1498074
TATACCG21100.016.1489755
TCTATAC35900.015.9537293
GTATATG47450.015.9074741
ACTATGC36500.015.6914768
TCTAGAC20000.015.5871983
TATACAG44100.015.4532145
CCTATAC25350.015.1574963
ACCTAAT43350.014.9006411
GTATATA79950.014.7062061
CCGTGCC7002.9958755E-914.4997189
Produced by FastQC (version 0.11.5)